国产亚洲高清在线精品不卡-国产亚洲精aa在线观看不卡-国产亚洲精彩视频-国产亚洲精品91-日本aⅴ日本高清视频影片www-日本aⅴ视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-6475B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

簡(jiǎn)要描述:CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-6475
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-08-29
  • 訪  問  量:1918

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

ATCC® Number:CRL-6475™  Price:$269.00
Designations:B16-F10

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:melanocyte
B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞


Source:Organ: skin
Strain: C57BL/6J
Disease: melanoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-6475

Applications:transfection host (technology from amaxa)

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase



CRL-6475















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 欧洲精品码一区二区三区免费看| 欧美日韩国产高清一区二区三区| 亚洲伊人色图| 亚洲高清不卡| 日韩一本二本三本| 青青久久国产| 欧美特级特黄a大片免费| 香港三级韩国三级日本三级| 色综合久久综合| 欧美一级片在线免费观看| 亚洲欧美日韩精品在线| 四虎影午夜成年免费精品| 香蕉成人国产精品免费看网站| 日日综合网| 日韩中文字幕在线| 性欧美午夜高清在线观看| 亚洲综合欧美在线| 色综合小说久久综合图片| 奇米影视888狠狠狠777九色| 日韩欧美亚洲一区精选| 亚洲精品xxxxx| 三级视频国产| 香港日本韩国三级| 亚洲一级色| 秋霞免费理论片鲁丝在线观看| 特级做a爰片毛片免费看| 亚洲一区精品视频在线| 亚洲婷婷综合中文字幕第一页| 亚洲成a人片在线看| 无人区理论片手机看片| 日本精品久久久久中文字幕2 | 欧美专区一区| 日本精品三级| 亚洲成人动漫在线| 日本污污网站| 午夜视频网| 色偷偷91久久综合噜噜噜噜| 亚洲美女aⅴ久久久91| 性色综合| 三级a黄| 小h片在线观看|