CRL-1711 SF9 昆蟲卵巢細胞

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細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養(yǎng)基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結直腸腺癌人經典小細胞肺癌細胞人小細胞肺癌細胞人結直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉錄病毒包裝穩(wěn)定細胞株小鼠神經細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導因子人乳腺癌細胞結腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導表達穩(wěn)定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產品

詳細介紹

CRL-1711 SF9 昆蟲卵巢細胞 的詳細介紹

ATCC^® Number:

CRL-1711™

Price:

$275.00

Designations:	Sf9
Depositors:	G Smith, C Cherry, MD Summers
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	mixed: adherent/suspension
Organism:	Spodoptera frugiperda （fall armyworm）
Morphology:	epithelial
Source:	Organ: ovary
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1983
Applications:	transfection host （Roche FuGENE® Transfection Reagents technology from amaxa）
Virus Susceptibility:	Nuclear polyhedrosis virus, Autographa californica St. Louis encephalitis virus
Age:	pupa
Gender:	female
Comments:	This line can be used to replicate baculovirus expression vectors. It is important to use the medium described below.
Propagation:	ATCC complete growth medium: Grace's Insect Medium with L-glutamine and supplemented with: 500 mg/L calcium chloride 2800 mg/L potassium chloride 3330 mg/L lactalbumin hydrolysate 3330 mg/L yeastolate 10% Heat-inactivated fetal bovine serum （previously tested for insect cell culture） Atmosphere: air, 100% Temperature: 28.0°C Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
Subculturing:	Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand （the latter is only preferable when working with larger flasks）. *Subction Ratio:** A subction ratio of 1:5 or greater is recommended Medium Renewal:* Every 2 to 3 days
Preservation:	Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
References:	22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda （Lepidoptera; Noctuidae）. In Vitro 13: 213-217, 1977. PubMed: 68913 23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453 23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519 32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separay cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050 32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063 32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863 32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109 32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508 33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802 33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793 33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda （Sf9） cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736 33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509

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